... against AML cells with FLT3 -ITD mutations in vitro and in vivo. Methods: The CCK-8 assay was used to analyze cell viability, and flow cytometry was used to detect cell apoptosis and differentiation. Western blotting and real-time polymerase chain reaction were used to examine the expression of certain...

... were isolated by differential centrifugation and identified. The effect of BMSCs-EVs on ALL cell proliferation and apoptosis was evaluated. The expression of miR-29b-3p in ALL cells and EVs was detected. The uptake of EVs by ALL cells was observed. The effect of miR-29b-3p on ALL cell proliferation...

... patients and CML cells was examined by quantitative real-time polymerase chain reaction. The levels of apoptosis-related proteins were determined using western blot. The relative cell survival rate and cell proliferation were determined using a CCK-8 assay and a bromodeoxyuridine (BrdU) assay, respectively...

... involved in association with the splenic micronodular pattern of the disease are the splenic hilar lymph nodes. The possibility that corticosteroids, when prescribed prior to splenectomy, cause histopathological and functional modulations, apoptosis, necrosis, tissue shrinkage, which may obscure...

... phenotype, based on the enhanced expression of p38-MAPK, EGR-1, p53, Bak, Bax, and ROS, and attenuated expression of Bcl-xL, suggesting increased sensitivity toward apoptosis. [email protected] 30 01 2021 8 06 2021 10 8 2021 © 2021 S. Karger AG, Basel 2021 Copyright / Drug...

... ]. However, to our knowledge, there have been no studies to investigate if SAMHD1 plays a role in apoptosis or autophagy. Our study indicated that SAMHD1 was not correlated with Bcl-2 family genes, IAP family genes, livin, or the autophagy marker LC3. In addition, from a clinical aspect, SAMHD1 had...

...Yang Liu; Xiaochen Yu; Junling Zhuang Objectives: To explore the effect of the β-adrenoreceptor signaling pathway on myeloma cells. Methods: The myeloma U266 cell line was treated with epinephrine and propranolol. Cell proliferation was analyzed by MTS assay. Apoptosis was detected by flow...

... (TKIs) on the expression of apoptosis-related genes (BCL-2 and death receptor family members) in chronic myeloid leukemia (CML) patients. Methods: Peripheral blood mononuclear cells from 32 healthy subjects and 26 CML patients were evaluated before and after treatment with imatinib mesylate (IM...

... far. The aim of the present study was to detect the methylation status of the TMS1 gene in K562 cells and the demethylation effect of As 2 O 3 on TMS1 as well as TMS1 apoptosis-associated protein Bcl-2/Bax and DNA methyltransferase (DNMT) expression. Methods: TMS1 mRNA expression in K562 cells...

...) and the potential protective effect of carvedilol. Methods: FeCl 3 was added to HUVEC culture. Iron entry into cells was monitored using fluorescent microscopic imaging, while the quantity of EMPs that was released was determined by flow cytometry. The apoptosis of HUVECs was assessed by annexin V/propidium iodide...

... HL-60 and vincristine-resistant HL-60/VCR cells. Methods: The effects of 14-3-3ζ siRNA on the growth and cell cycle progression of HL-60 and HL-60/VCR cells were determined. The effect of 14-3-3ζ siRNA on topotecan (TPT)-induced apoptosis was evaluated by several assays. Results: Compared to HL-60...

... and apoptosis under certain experimental conditions. However, the detailed mechanism by which bufalin induces the apoptosis of acute promyelocytic leukemia cells is largely unexplored. Methods: The acute promyelocytic leukemia cell line NB4 was treated with bufalin, then the proliferation was evaluated by cell...

...Wasinee Kheansaard; Prapaporn Panichob; Suthat Fucharoen; Dalina I. Tanyong Background/Aim: β-Thalassemia/hemoglobin E (β-thal/HbE) is a common hereditary anemia in Thailand. Ineffective erythropoiesis due to apoptosis and decreased lifespan of circulating thalassemic red blood cells are the major...

... of our study was to investigate the mechanism involved in the pathogenesis of osteonecrosis. We examined the apoptosis of circulating endothelial progenitor cells in MM subjects before and after BP treatment and in osteonecrosis patients using a flow-cytometric analysis. Our data showed an increase...

.... In this study, the ability of short-interfering RNA (siRNA) to downregulate hTERT expression and its correlation with cell growth and apoptosis in the promyelocytic cell line HL-60 was evaluated. Materials and Methods: hTERT siRNA was designed and transfected to HL-60. hTERT mRNA expression, cell proliferation...

... for identifying the group of patients who require intensive treatment. The aim of this study was to assess whether the rate of apoptosis caused by purine analogues differs between patients with better or worse prognostic factors. The experiments were preformed in cultures of blood and bone marrow obtained from...

...I. Vermes; C. Haanen; D J. Richel; M.R. Schaafsma; E. Kalsbeek-Batenburg; C.P.M. Reutelingsperger It has been reported that cultured peripheral B lymphocytes of chronic lymphocytic leukemia (B-CLL) patients show a high degree of apoptosis (programmed cell death). Till now, no data exist about...

...J. Thiele; J. Lorenzen; B. Manich; H.M. Kvasnicka; T.K. Zirbes; R. Fischer There is general agreement that apoptosis (programmed/physiological cell death) plays an important role in regulating normal hematopoiesis and that so-called naked nuclei (NN) present end stages of megakaryopoiesis. Using...

... relapse was well controlled by low doses of steroids alone, and the patient’s condition stabilized. Considering the recent data that corticosteroids can induce apoptosis of CLL cells, the above clinical course suggests that augmented glucocorticoid production from the tumor may have suppressed disease...

... such as 1, 2-dimethyl-3-hydroxypyridin-4-one (L1, CP20, deferiprone) and l,2-diethyl-3-hydroxypyridin-4-one (CP94). New tissue culture approaches towards understanding the mechanisms of neutropenia, cytostasis and apoptosis induced by chelators as well as the relative rates of inhibition of non-haem-iron...