The hypothesis that human red cells contain activity of ribulose-1,5-diphosphate (Ru-1,5-P2) oxygenase, an enzyme that catalyzes the synthesis of phosphoglycolate, was examined. The putative Ru-1,5-P2 oxygenase was partially purified from human red cells using DE-52 chromatography and (NH4)2SO4 fractionation by monitoring Ru-1,5-P2-dependent 3-phosphoglycerate formation. The synthesis of [32P]phosphoglycolate from [1-32P]Ru-1,5-P2 was attempted in the presence of the partially purified preparation of the provisional Ru-1,5-P2 oxygenase. There was no formation of radioactive phosphoglycolate even under 100% oxygen gas, indicating the absence of this enzyme activity in human red cells. Together with our previous report that glycolate kinase in human red cells is not responsible for the synthesis of phosphoglycolate in vivo, these studies raise the questions whether there is actually phosphoglycolate in red cells as well as whether novel pathways for its synthesis exist.

This content is only available via PDF.
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.