We have studied the amount and intracellular distribution of erythroblastic alkaline and acid phosphatase, nonspecific esterase, and N-acetyl-β-glucosaminidase in 50 patients with acquired dyserythropoiesis. 19 morphologically normal bone marrow smears served as controls. Alkaline phosphatase was found in all controls (mean 9.4% of erythroblasts). The percentage of positive erythroblasts in pathological conditions varied greatly from absence to over 70%. Acid phosphatase and N-acetyl-β-glucosaminidase were positive in pernicious anaemia and in acute erythraemic myelosis. Nonspecific esterase was only detected in a case of erythraemic myelosis. This case also showed a faint metachromasia with the dye azure A.

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