Normal human neutrophils, incubated with 0.2 mg/ml opsonized zymosan particles, were found to lyze human (H), ox (O) and chicken (C) red blood cell (RBC) targets as determined by the 51Cr release assay. The susceptibility to the lysis of the different target cells was HRBC < ORBC < CRBC. An intact neutrophil metabolic burst was essential for the cytotoxic event, since neutrophils from a patient with chronic granulomatous disease failed to kill all three target cells. HRBC and ORBC destruction was prevented by catalase and unaffected by azide, suggesting the requirement of hydrogen peroxide alone in the lethal hit. CRBC destruction was abolished by catalase and azide, suggesting the involvement of the myeloperoxidase-hydrogen peroxide system. Thus, different neutrophil cytolytic systems may become operative and may vary in their efficiency depending on the type of target cells.

Keywords:
Cytotoxicity, Neutrophils, Phagocytosis
This content is only available via PDF.
© 1984 S. Karger AG, Basel
1984
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.