Dogs were chronically irradiated (10 R/day; 0–400 R) or acutely irradiated (20 R/min; 0–400 R) with 60Co γ-rays. Sera were collected and assayed for colony-enhancing activity by a double-layer agar cloning technique. When test sera alone were incorporated in the feeder layers, no colony-forming units-granulocyte/macrophage formation occurred; the addition of peripheral blood leukocytes to such test sera-containing feeder layers resulted in formation of colonies, the number of which was directly related to the cumulative radiation dose. Serum-enhancing factor activity indirectly increased colony formation by stimulating feeder layer leukocytes to generate increased levels of colony-stimulating activity. Levels of serum-enhancing activity were higher at similar dose levels following acute gamma irradiation then following chronic irradiation.

Keywords:
CFU-GM, CSA, Dogs, Enhancing effects, γ-Irradiation
This content is only available via PDF.
© 1984 S. Karger AG, Basel
1984
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.