In order to evaluate the validity of 3H-diisopropyl fluorophosphate (DFP) as a label for neutrophil kinetic studies, the blood and skin chamber kinetics of autologous neutrophils doubly labeled with 3H-DFP and 32P-DFP were determined in 6 normal subjects. Blood disappearance time and skin chamber accumulation of label was slightly but significantly decreased using 3H values, indicating mild elution of the isotope from the cells. The degree of elution was small in comparison to the previously observed rate of elution of 32P-DFP and suggests that 3H-DFP is an acceptable alternative label.
Keywords:Neutrophil, Granulocyte, Radioisotope, Diisopropylfluorphosphate, Blood, Skin chamber, Kinetics, In vivo, Autologous
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© 1982 S. Karger AG, Basel
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