40 patients, 15 females and 25 males, with biopsy-proven non-Hodgkin’s lymphoma within the age range of 2–16 years were included in this study. The mean serum zinc level in 38 untreated patients was 78.95 ± 18.80 μ g% and significantly lower than in controls (p < 0.001). The mean serum copper level in 40 untreated patients was 205.32 ± 49.44 μ g% and significantly higher than in controls (p < 0.001). The mean copper/zinc ratio in these patients was 2.75 and was found to be significantly higher than in controls (p < 0.01). Copper levels were lower during remission (p < 0.05). Our data indicate that serum copper levels and the copper/zinc ratio might be indicators for disease activity in children with non-Hodgkin’s lymphoma.