The presence of in vitro colony-forming units (CFUc) was demonstrated in leukocyte suspensions collected by continuous flow leukaphereses performed serially in dogs. The CFUc yield of one leukapheresis was usually between 104 and 106. Higher yields were obtained in the 2nd to 5th leukaphereses than in the first one in the same dog. The number of CFUc collected during one leukapheresis was about four times the number of CFUc present in the circulating blood at the beginning of the leukapheresis. After a diminution during leukapheresis, the circulating CFUc pool was restored in 2 or 3 days with an increase in the ratio of CFUc to mononuclear cells. These results indicate that CFUc entered the circulating pool during and after leukapheresis and that the population size regulation of canine-circulating CFUc is different from that of the bulk of the mononuclear leukocytes.

Keywords:
Leukapheresis, Canine leukocytes, Cell culture, Colony formation
This content is only available via PDF.
© 1978 S. Karger AG, Basel
1978
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.