Many bovine leukemic lymphocytes produce virus particles when kept in survival cultures in Eagle’s Minimum Essential Medium supplemented with 20% of inactivated fetal calf serum. Virus particles equilibrate at a density of 1.16 g/ml in sucrose gradients and at a density of 1.12 g/ml in metrizamide gradients. Simultaneous detection tests show that a high molecular weight RNA-reverse transcriptase complex exists in these viruses. Hybridizations between total RNA from bovine leukemic lymphocytes and C-DNA prepared in various known RNA oncogenic viruses show that the virus associated with bovine leukosis is unrelated to RLV, SSV-1, MSV-Ki, FSV-Ga and FLV-Ri.

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