In 6 cases initially showing a symptomatology of erythroleukaemia, the proliferation of erythroblasts was studied by combined Feulgen cytophotometry and autoradiography after labelling with 3H-TdR in vivo. A proliferation disturbance was observed consisting of an accumulation of diploid and unlabelled cells and a decreased proportion of cells in S. This defect occurred within the basophilic cell compartment in one case and within the early polychromatic cell compartment in all 6 cases. The results indicate the existence of out-of-cycle cells, which may be responsible for the inefficacy for erythropoiesis in this disease. The defect was present in all 4 cases with acute erythroleukaemia. In one of the two cases showing no deterioration of the physical and haematological condition over a period of years, an additional defect was observed, consisting of a striking accumulation of tetraploid unlabelled cells. Therefore, the technique used may be suitable as a diagnostic tool for evaluation of new types of ineffective erythropoiesis.

Keywords:
Autoradiography, Cytophotometry, DNA synthesis, Erythro-blasts, Erythroleukaemia, G0 cells, Ineffective erythropoiesis
This content is only available via PDF.
© 1975 S. Karger AG, Basel
1975
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.