In comparison to other criteria of iron status, higher degrees of association have been computed between the diffuse Prussian blue reaction of the bone marrow macrophages and intestinal ferrous iron absorption, when test doses of 0.56 mg, 50 mg in solution, and 50 mg in quick-release capsules were given to fasting iron-replete controls and iron-deficient subjects in 59Fe absorption whole-body retention tests. The variability of the results increased with rise of the test doses. Antecedent oral iron substitution and unallowed intake of food depressed ferrous iron absorption considerably.

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