Nucleoli of lymphocytes from peripheral blood can be demonstrated by methylene blue staining according to the method of Pischγnger modified by Stockinger and Kellner. allows their classification in macro and polynucleolar lymphocytes.The application of toluidine blue staining according to lenert made it possible to differentiate peripheral lymphocyte populations of children without any haematological diseases, treated for longer periods with corticosteroids or cytostatic drugs (immunosuppressive therapy). Compared with control groups (normal persons, children with bacterial infections treated with antibiotics, partly for a short period with corticosteroids), the percentage of macronucleolar lymphocytes increased while polynucleolar types were diminishing. An interpretation is not yet possible.

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