In this study, we determined the allele and genotype frequencies of vascular endothelial growth factor (VEGF) G+405C, C–460T, C+936T and C–2578A single nucleotide polymorphisms (SNPs) in 32 patients affected by mantle cell lymphoma (MCL) and 58 healthy controls. Real-time PCR combined with melting curve analysis was used for the determination of SNP alleles. A significant difference in the allele frequency of VEGF C–460T and C+936T SNPs in MCL and healthy cases was not observed. On the contrary, VEGF G+405C and C–2578A SNP allele distribution was significantly lower in the patient group than among normal controls (p = 0.014, p = 0.001). This observation suggests that further investigation is warranted, both in vitro and in a larger series of patients, to further examine the role of VEGF polymorphisms in the pathogenesis of MCL. In addition, the use of quantitative real-time PCR combined with a melting curve analysis method in the detection of the 4 VEGF SNPs may have the potential to replace older and more time-consuming PCR-RFLP methods and bears further investigation.

Keywords:
Mantle cell lymphoma, Polymorphism, Single nucleotide polymorphism, Vascular endothelial growth factor
© 2009 S. Karger AG, Basel
2009
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