The effect of the underlying disease and chemotherapy on megakaryopoiesis has not been extensively studied in children with acute lymphoblastic leukemia (ALL) during and at the end of therapy. Using a serum-free assay, we assessed the megakaryocyte (Mk) colony formation in vitro from bone marrow mononuclear cells of 25 children with ALL during chemotherapy and shortly after the cessation of it. Twelve children with solid tumors without bone marrow involvement and cord blood from 10 full-term normal vaginal deliveries were used as controls. A significant reduction in the number of Mk colonies was observed at diagnosis of ALL, and Mk colony formation remained lower than controls throughout the different phases of leukemia treatment. Our study suggests that defects in megakaryopoiesis of children with ALL in long-term remission may persist during chemotherapy and at least shortly after the end of it.

1.
Dimitriou H, Perdikoyanni C, Lydaki E, Stiakaki E, Bolonaki I, Kalmantis Th, Kalmanti M: Erythropoiesis and myelopoiesis in childhood leukemia and solid tumors. Int J Pediatr Hematol Oncol 1999;6:243–250.
2.
Schipper LF, Brand A, Reniers N, Melief CJ, Willemze R, Fibbe WE: Differential maturation of megakaryocyte progenitor cells from cord blood and mobilized peripheral blood. Exp Hematol 2003;31:324–330.
3.
Majka M, Baj-Krzyworzeka M, Kijowski J, Reca R, Ratajczak J, Ratajczak MZ: In vitro expansion of human megakaryocytes as a tool for studying megakaryocytic development and function. Platelets 2001;12:325–332.
4.
Cox CV, Killick SB, Patel S, Elebute MO, Marsh JC, Gordon-Smith EC, Gibson FM: In vitro proliferation and differentiation of megakaryocytic progenitors in patients with aplastic anemia, paroxysmal nocturnal hemoglobinuria, and the myelodysplastic syndromes. Stem Cells 2000;18:428–434.
5.
Won JH, Cho SD, Park SK, Lee GT, Baick SH, Suh WS, Hong DS, Park HS: Thrombopoietin is synergistic with other cytokines for expansion of cord blood progenitor cells. J Hematother Stem Cell Res 2000;9:465–473.
6.
Sakaguchi M, Kawakita M, Matsushita J, Shibuya K, Koishihara Y, Takatsuki K: Human erythropoietin stimulates murine megakaryopoiesis in serum-free culture. Exp Hematol 1987;15:1028–1034.
7.
Broudy VC, Lin NL, Kaushansky K: Thrombopoietin (c-mpl ligand) acts synergistically with erythropoietin, stem cell factor, and interleukin-11 to enhance murine megakaryocyte colony growth and increases megakaryocyte ploidy in vitro. Blood 1995;85:1719–1726.
8.
Kimura H, Burstein SA, Thorning D, Powell JS, Harker LA, Fialkow PJ, Adamson JW: Human megakaryocytic progenitors (CFU-M) assayed in methylcellulose: Physical characteristics and requirements for growth. J Cell Physiol 1984;118:87–96.
9.
Hirt A, Schmid AM, Luethy AR, Gugler E, Wagner HP: Recovery of megakaryocytopoiesis in vitro in children with acute lymphoblastic leukemia during induction of remission. Exp Hematol 1990;18:37–40.
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