Objectives: Telomerase activity can be detected in up to 90% of urothelial carcinomas (UC). Telomerase activity can also be detected in urinary tract cytology (UTC) specimens and indicate an increased risk of UC. We evaluated the performance of a commercially available antibody that putatively binds the telomerase reverse transcriptase (hTERT) subunit on 500 UTC specimens. Study Design: Unstained CytospinTM preparations were created from residual urine specimens and were stained using the anti-hTERT antibody (SCD-A7). Two algorithms were developed for concatenating the hTERT result and cytologic diagnosis: a “no indeterminates algorithm,” in which a negative cytology and positive hTERT result are considered positive, and a “high-specificity algorithm,” in which a negative cytology and positive hTERT result are considered indeterminate (and thus negative for comparison to the gold standard). Results: The “no indeterminates algorithm” and “high-specificity algorithm” yielded a sensitivity of 60.6 and 52.1%, a specificity of 70.4 and 90.7%, a positive predictive value of 39.1 and 63.8%, and a negative predictive value of 85.0 and 85.8%, respectively. Conclusions: A positive hTERT result may identify a subset of patients with an increased risk of high-grade UC (HGUC) who may otherwise not be closely followed, while a negative hTERT immunocytochemistry result is associated with a reduction in risk for HGUC.

Urinary tract carcinoma (UC) is a treatable but burdensome disease that requires continual surveillance because of the high risk of recurrence [1, 2]. The visualization of tumors during cystoscopy and ureteroscopy is considered the gold standard method of detection but is an invasive procedure that is time-consuming, costly, and uncomfortable for the patient. Urinary tract cytology (UTC) is a useful, noninvasive adjunct for surveillance because of its high specificity for the detection of high-grade urothelial carcinoma (HGUC) [3, 4]. Unfortunately, UTC has limited sensitivity for the detection of HGUC and poor sensitivity and specificity for the detection of low-grade urothelial neoplasms, such as low-grade urothelial carcinoma (LGUC) [5, 6]. A number of noninvasive, ancillary tests have been developed and reported in the literature [7, 8]. Some, such as FISH, are used in conjunction with UTC, while others are used independently of UTC results; however, none of these tests have gained universal acceptance.

Studies have detected telomerase activity in up to 90% of UC. Furthermore, telomerase activity can be detected in UTC and indicates an increased risk for having UC [9-13]. hTERT (telomerase reverse transcriptase) is the catalytic subunit component of the telomerase ribonucleoprotein complex. The majority of UC (60–80%) have mutations in the TERT promoter, which can also be found in some histologic variants of UC [14-18]. A small study of 101 cell blocks created from urinary sediment found that hTERT immunostaining had a sensitivity of 84.8% and specificity of 65.2% for the detection of UC [19].

In this study, we investigate the performance of a commercially available antibody that putatively binds hTERT. To do so, we performed immunocytochemistry (ICC) and blindly interpreted the result on 500 consecutive UTC specimens submitted to our laboratory.

Specimen Cohort

The institutional review board approved this study and provided a consent waiver. 500 consecutive residual urine specimens from 474 unique patients submitted to the cytopathology laboratory for clinical diagnosis were utilized, with specimens only being excluded if insufficient residual material to create an experimental preparation remained following the rendering of a clinical diagnosis. Specimens with a volume of ≥20 mL were stored at 2–8°C and subsequently processed in batches every 2–3 days.

Clinical specimens were prepared using a minimum of 25 mL of fresh urine and processed using the BD SurePath® liquid-based preparation. The clinical diagnosis was rendered by 1 of 5 cytopathology-boarded pathologists in the Johns Hopkins Hospital (JHH) Division of Cytopathology.

Diagnoses were made using the Johns Hopkins Template for Reporting Urinary Tract Cytology (JHHT) because the Paris System for Reporting Urinary Tract Cytology had not yet been established at JHH during this study period [20-22]. The JHHT utilized a malignant category, “high-grade urothelial carcinoma” (HGUC), a benign category, “no urothelial atypia or malignancy” (NUAM), a low-risk indeterminate category, “atypical urothelial cells of undetermined significance” (AUC-US), and a high-risk indeterminate category, “atypical urothelial cells, cannot exclude HGUC” (AUC-H).

Experimental Slide Preparation

Specimens were transferred and centrifuged in 50-mL centrifuge tubes at 470 g for 10 min at 2–8°C. The supernatant was discarded, and the cell pellet resuspended in 10 mL phosphate-buffered saline, pH 7.4, prior to being centrifuged at 470 g for 10 min at 2–8°C. This step was repeated if the cell pellet was found to contain large proteinaceous material upon inspection. Cell pellets were then resuspended in 10 mL alcohol containing carbowax (PEG) fixative (ShandonTM CytospinTM Collection Fluid, Thermo ScientificTM) and centrifuged at 470 g for 10 min at 2–8°C.

The supernatant was discarded, and fixed cell pellets were resuspended in a 500-µL to 1-mL fixative solution. Specimen cells are affixed to slides by adding the cell suspensions to a CytospinTM funnel attached via a cytoclip to positively charged glass microscope slide and centrifuged at 113 g (1,000 rpm) for 4 min with a low acceleration setting using a CytopsinTM 4 Cytocentrifuge (Thermo ScientificTM). Three slides were prepared using the thin-layer liquid-based cytology preparation for each patient specimen. Slides were subsequently stored and held in slide boxes at 2–8°C until ICC was carried out.

Immunocytochemistry

Anti-hTERT antibody (SCD-A7) is a mouse monoclonal antibody produced as a tissue culture supernatant and supplied in phosphate-buffered saline with carrier protein, containing 0.05% ProClin® 300 as a preservative (Sienna Cancer Diagnostics Ltd., Melbourne, Australia, used at 5.4–6.6 µg/mL).

Slides were removed from the 2–8°C storage and placed onto the Ventana BenchMark Ultra automated staining platform (Ventana Medical Systems Inc., Tucson, AZ, USA). Slides were treated with heat-induced epitope retrieval performed as follows: Ultra Cell Conditioning (Ultra CC1, pH 8.5) retrieval buffer for 8 min at 95°C. Slides were then incubated with the diluted (1/11) anti-hTERT antibody for 44 min at 37°C. Nonspecific antibody binding was blocked by incubating cytology specimens with OptiView peroxidase inhibitor containing 3% hydrogen peroxidase solution and DISCOVERY antibody diluent (Ventana Medical Systems Inc., Tucson, AZ, USA) for 8 min. For hTERT immunocytochemical staining, the OptiView DAB IHC detection kit (Ventana Medical Systems Inc., Tucson, AZ, USA) was used which makes use of a cocktail of secondary antibodies that locate the bound primary antibody. The slides were incubated with OptiView HQ universal linker and OptiView HRP multimer for 8 min at 37°C. The secondary antibody cocktail was recognized by an enzyme-bound, tertiary antibody that was visualized with hydrogen peroxide substrate and the 3,3′-diaminobenzidine tetrahydrochloride chromogen, which produces a brown precipitate that is readily detected by light microscopy. Slides were counterstained with hematoxylin II (Ventana Medical Systems Inc., Tucson, AZ, USA) and then mounted.

Experimental Slide Interpretation

Interpretations were performed by 1 board-certified cytopathologist (C.J.V.). The interpretation of hTERT ICC was determined based on preliminary observations made during previous antibody optimization runs and recommendations made by Sienna Cancer Diagnostics, which were finalized prior to the blind reading of the experimental slides and are described below.

The cutoff for determining a positive hTERT ICC result was nuclear staining of: (1) at least 1 urothelial cell with atypical cytomorphology or (2) at least 2 cytomorphologically benign-appearing urothelial cells. Cytoplasmic staining was not regarded as a positive result and its presence did not invalidate a positive nuclear stain. The following nonurothelial components consistently stained positively: bacteria, lymphocytes, and neutrophils. Occasional nuclear staining of mature squamous cells could be identified and was not regarded as a positive result. Positive-staining granular debris of unknown origin was sometimes present in the background and was not considered a positive result. The hTERT ICC result was considered uninterpretable if: (1) less than 5 urothelial cells were present for evaluation or (2) any positive-staining nonurothelial component interfered with the interpretation of the urothelial cell component. Cells that could not be definitively determined to be of squamous versus urothelial origin were considered to be urothelial cells (Fig. 1).

Fig. 1.

Examples of hTERT interpretation. a Numerous malignant cells are seen, more than half demonstrating positive nuclear staining. The cells have recognizable and intact cytoplasm on the counterstain and are morphologically compatible with urothelial cells; thus, it is appropriate to interpret them as hTERT positive. The presence of cytoplasmic staining in some of these cells does not invalidate the result. b Two cells (lower left) have nuclear staining and recognizable, intact cytoplasm. The cells are morphologically compatible with urothelial cells; a mature squamous cell (upper right) acts as an internal negative control. c A cluster of bacteria stain positively by hTERT and approximate the size of a urothelial cell nucleus. The stain was not interpreted as positive if a cell’s cytoplasm was not clearly seen. d–f Numerous positive-staining bacteria are present in the background, stuck to mature squamous cells. In cases where positive-staining inflammatory cells and/or bacteria obscured the preparation, the test was considered noninterpretable. f Despite positive-staining bacteria at the bottom of the field, a positive urothelial cell is clearly seen in the center of the field. In such instances, cases could be interpreted as positive.

Fig. 1.

Examples of hTERT interpretation. a Numerous malignant cells are seen, more than half demonstrating positive nuclear staining. The cells have recognizable and intact cytoplasm on the counterstain and are morphologically compatible with urothelial cells; thus, it is appropriate to interpret them as hTERT positive. The presence of cytoplasmic staining in some of these cells does not invalidate the result. b Two cells (lower left) have nuclear staining and recognizable, intact cytoplasm. The cells are morphologically compatible with urothelial cells; a mature squamous cell (upper right) acts as an internal negative control. c A cluster of bacteria stain positively by hTERT and approximate the size of a urothelial cell nucleus. The stain was not interpreted as positive if a cell’s cytoplasm was not clearly seen. d–f Numerous positive-staining bacteria are present in the background, stuck to mature squamous cells. In cases where positive-staining inflammatory cells and/or bacteria obscured the preparation, the test was considered noninterpretable. f Despite positive-staining bacteria at the bottom of the field, a positive urothelial cell is clearly seen in the center of the field. In such instances, cases could be interpreted as positive.

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Gold Standard Comparison

To compare the cytologic diagnoses and test interpretation against a gold standard result, we considered results from the patient’s concurrent cystoscopy, concurrent and subsequent tissue biopsies, and subsequent UTC specimen results. The follow-up period ranged from 6 to– 12 months depending on the time at which the specimen was collected and the time at which the slides were stained and clinical data were obtained. Either a positive diagnosis for a urothelial carcinoma (HGUC or LGUC) on biopsy or a diagnosis of HGUC with UTC were considered as a positive follow-up regardless of the cystoscopy result. In the absence of a follow-up biopsy or positive UTC specimen, a positive cystoscopy result was considered as a positive follow-up. A positive cystoscopy was considered negative if a follow-up biopsy occurred and was negative for UC.

Study Cohort

The study cohort contained 410 specimens in which the test result was considered interpretable (Fig. 1). 297 (72.4%) of these specimens had an associated concurrent cystoscopy or follow-up specimen (as defined in the Methods section) as a gold standard for comparison. Associated patient information is summarized in Table 1; because these specimens were de-identified, some specimens may represent repeat specimens from the same patient, which is not accounted for in the demographic data.

Table 1.

Characteristics of the 297 specimens with an interpretable hTERT immunocytochemistry result

Characteristics of the 297 specimens with an interpretable hTERT immunocytochemistry result
Characteristics of the 297 specimens with an interpretable hTERT immunocytochemistry result

Performance of Cytologic Diagnosis

Among the cohort of 297 specimens with a gold standard for comparison, the distribution of initial cytology diagnoses was as follows: 190 NUAM, 80 AUC-US, 16 AUC-H, and 11 HGUC. Given our institution’s high rate of HGUC among AUC-H diagnoses, NUAM and AUC-US diagnoses were considered negative, and HGUC and AUC-H were considered positive for comparison to the gold standard. The specificity of cytology within this test cohort for any urothelial carcinoma, regardless of grade, was 97.8% and the sensitivity was 31.0% (Fig. 2).

Fig. 2.

Performance of cytology alone compared to the study’s gold standard.

Fig. 2.

Performance of cytology alone compared to the study’s gold standard.

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Test Performance Using the “No Indeterminates” Algorithm

In order to interpret the test result in the context of the cytologic result, 2 possible algorithms were applied. The “no indeterminates” algorithm interprets NUAM specimens with a positive hTERT result as an overall positive result (Table 2). When this algorithm was applied and the overall result compared to the gold standard, the specificity of the test was 70.4% and the sensitivity 60.6% (Fig. 3, 4).

Table 2.

Final test interpretation for comparison to gold standard based on concatenation of urinary cytology specimen clinical diagnosis and hTERT result

Final test interpretation for comparison to gold standard based on concatenation of urinary cytology specimen clinical diagnosis and hTERT result
Final test interpretation for comparison to gold standard based on concatenation of urinary cytology specimen clinical diagnosis and hTERT result
Fig. 3.

Flowchart detailing the gold standard follow-up for each specimen, with associated combined hTERT and cytology results according to the “no indeterminates” and “high-specificity” algorithms.

Fig. 3.

Flowchart detailing the gold standard follow-up for each specimen, with associated combined hTERT and cytology results according to the “no indeterminates” and “high-specificity” algorithms.

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Fig. 4.

Overall performance of cytology plus hTERT immunocytochemistry (ICC) compared to the study’s gold standard. The “no indeterminates” algorithm considers a benign cytology specimen with a positive hTERT ICC result as an overall positive result for comparison to the gold standard.

Fig. 4.

Overall performance of cytology plus hTERT immunocytochemistry (ICC) compared to the study’s gold standard. The “no indeterminates” algorithm considers a benign cytology specimen with a positive hTERT ICC result as an overall positive result for comparison to the gold standard.

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Test Performance Using the “High-Specificity” Algorithm

The second algorithm (“high-specificity” algorithm) interprets NUAM specimens with a positive hTERT result as “atypical” and considered negative for comparison to the gold standard (Table 2). In this context, the specificity of the test was 90.7% and the sensitivity 52.1% (Fig. 5).

Fig. 5.

Overall performance of cytology plus hTERT immunocytochemistry (ICC) compared to the study’s gold standard. The “high-specificity” algorithm considers a benign cytology specimen with a positive hTERT ICC result as indeterminate and thus as an overall negative result when compared to the gold standard.

Fig. 5.

Overall performance of cytology plus hTERT immunocytochemistry (ICC) compared to the study’s gold standard. The “high-specificity” algorithm considers a benign cytology specimen with a positive hTERT ICC result as indeterminate and thus as an overall negative result when compared to the gold standard.

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Test Performance for LGUC

The study cohort contained 24 specimens in which the patient was subsequently diagnosed with LGUC on tissue biopsy. The cytologic diagnosis was NUAM for 9 specimens and AUC-US for 15 specimens. Among these, only 3 specimens diagnosed as NUAM were hTERT ICC positive, and almost half (7/15) of those diagnosed as AUC-US were hTERT ICC positive. These results amount to a sensitivity of 41.6% according to the “no indeterminates” algorithm and a sensitivity of 29.2% according to the “high-specificity” algorithm.

Predicting Test Impact in Instances Cystoscopy Was Not Performed or Negative

Assuming patients with positive cystoscopies would receive a closer follow-up regardless of cytologic diagnosis and those with HGUC or AUC-H diagnoses would receive a closer follow-up regardless of cystoscopy diagnosis, we investigated the impact of hTERT ICC results on those “lower-risk” patients who may not otherwise receive close follow-ups.

22 patients had no concurrent cystoscopy specimen; 3 were diagnosed with AUC-US and 19 with NUAM (22 total AUC-US or NUAM). 199 patients had negative concurrent cystoscopies; 46 were diagnosed with AUC-US, 143 with NUAM, 5 with AUC-H, and 5 with HGUC (189 total AUC-US or NUAM). Thus, 211 patients would not have been put into a higher risk category based on cytology and cystoscopy results alone. With hTERT test utilization in this subset of patients, 11 of the 211 would have received an additional follow-up. Two of these 11 were ultimately diagnosed with HGUC (18.2% prevalence compared to 2.8% prevalence in the larger subset). Of the remaining 200 patients lacking any positive result by hTERT ICC, cytology, and cystoscopy, 4 were ultimately diagnosed with HGUC (2.0% prevalence compared to 2.8% prevalence in the larger subset). Therefore, while the test lacks some specificity for low-risk patients, a negative test is reassuring in this setting.

Sensitivity for the test was significantly higher than cytology alone, especially in the ability to detect LGUC. The sensitivity of cytology alone is higher when low-grade lesions are not considered; this is an important tenant of the Paris System for Reporting Urinary Tract Cytology, given the extremely low sensitivity and specificity for detecting low-grade lesions by UTC. The additional detection of LGUC lesions contributed to 7 of 15 additional “true-positive” results by the “high-specificity” algorithm and 10 of 21 additional true-positive results by the “no indeterminates” algorithm.

Concerning the biopsy-established LGUC cohort, a positive hTERT result correlated to the gold standard with a 42% sensitivity using the “no indeterminates” algorithm, or 29% using the “high-specificity” algorithm. This small difference is due to a positive hTERT ICC result in 2 specimens with a cytologic diagnosis of NUAM; the majority of hTERT ICC positive specimens with LGUC follow-up had cytologic diagnoses of AUC-US. Low-grade lesions sometimes contain recognizable atypia but have a decreased tendency to shed into the urine as compared to HGUC lesions [6]. The absence of cytologic atypia in the NUAM specimens may indicate that these specimens may not have contained any lesional cells and, thus, were more likely to be hTERT ICC negative. The majority (9/10) of hTERT ICC positive specimens from patients with LGUC were voided urines. These findings suggest suggests that LGUC can shed into voided urine without requiring instrumentation for dislodgement and that the cytologic atypia associated with these lesions was not severe enough to result in a diagnosis of AUC-H or HGUC.

This current study’s design inherently suffers due to the potential for “false-positives,” in which a test may detect a lesion prior to the gold standard method [23]. Reports have shown this pitfall in other studies of UTC ancillary tests, and even positive cytomorphologic diagnoses may precede tissue biopsy confirmation by several months to years. This phenomenon is likely due to multiple factors, including the fact that cystoscopy at best has a sensitivity of 90%, carcinoma in situ may be difficult to identify on cystoscopy, and dysplastic or early neoplastic changes may harbor detectable alterations. Some of the “false-positive” hTERT ICC results are instances in which cytology, hTERT ICC, and initial cystoscopy were all positive, yet the follow-up biopsy was negative for UC. In these circumstances, it is expected that some biopsies did not sample the relevant lesion, or that carcinoma in situ cells detached from the biopsy area, leaving only denuded tissue.

There were 9 specimens for which the “high-specificity” algorithm was positive, but the cystoscopy was initially reported as negative. Subsequent follow-up biopsy revealed HGUC or carcinoma in situ in 7 of those cases. This finding indicates that at least some of the “false-positive” hTERT ICC results correctly predicted either a higher likelihood for carcinoma (that was perhaps missed on initial cystoscopy) or for the development of carcinoma (identified on later cystoscopy).

Regarding the hTERT ICC test, there are 2 limitations that prevent higher levels of sensitivity from being achieved. The first is that with exfoliative UTC specimens, the ability to detect cancer depends on those cells having been shed into the urine at the time of sample collection. Secondly, a small proportion of UC do not have alterations in telomerase activity. Indeed, in the current study, 2 of 11 specimens with a cytologic diagnosis of HGUC were hTERT ICC negative, which corresponds to reports that telomerase activity is detectable in up to 90% of UC.

An additional limitation of hTERT ICC is that non-urothelial cells may be positive, or are expected to be positive, and this may result in either an obscured field or misinterpretation of the cells as urothelial cells (false-positive result). hTERT ICC positivity is consistently observed in bacteria, neutrophils, and lymphocytes. This positivity, however, may be beneficial as it provides positive internal controls. The interpretation of hTERT ICC differs from most clinically utilized antibodies and likely requires experienced interpretation (Fig. 6). Other pitfalls are similar to those seen in slide-based, ancillary assays. For instance, the cellular material present on the hTERT ICC slide may not be representative of that present on the slide created for clinical diagnosis; an atypical or suspicious diagnosis may be rendered due to rare, atypical cells; if these cells are not also present on the slide created for ICC, the ICC result would be noncontributory.

Fig. 6.

Examples of hTERT staining. a A cluster of 3 cells (top center) stains positive; note the negative cell on the right side of the field. The corresponding diagnostic specimen was called “negative for urothelial atypia or malignancy”; the patient was subsequently found to have a low-grade urothelial neoplasm. b A cluster of atypical urothelial cells, the majority of which stain positive. The corresponding diagnostic specimen was called “atypical urothelial cells of uncertain significance”; the patient was subsequently found to have high-grade urothelial carcinoma. c–h Additional specimens in which hTERT was interpreted to be positive. i An example of a specimen containing high-grade urothelial carcinoma cells in which hTERT was negative.

Fig. 6.

Examples of hTERT staining. a A cluster of 3 cells (top center) stains positive; note the negative cell on the right side of the field. The corresponding diagnostic specimen was called “negative for urothelial atypia or malignancy”; the patient was subsequently found to have a low-grade urothelial neoplasm. b A cluster of atypical urothelial cells, the majority of which stain positive. The corresponding diagnostic specimen was called “atypical urothelial cells of uncertain significance”; the patient was subsequently found to have high-grade urothelial carcinoma. c–h Additional specimens in which hTERT was interpreted to be positive. i An example of a specimen containing high-grade urothelial carcinoma cells in which hTERT was negative.

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In evaluating the hTERT ICC, we have modeled how it may provide useful information when used in conjunction with cytology. The test may be most helpful in circumstances where a patient has a low-risk indeterminate UTC diagnosis (AUC-US) and a negative or absent cystoscopy result. A positive hTERT result may identify a small subset of patients with an increased risk of HGUC who may otherwise not be closely followed, while a negative hTERT ICC result is associated with a slight reduction in risk for HGUC.

The authors wish to thank Mr. Minesh Lalla for his excellent technical support and advice during this study and Dr. Dorothy Rosenthal for her expertise and advice during optimization studies that preceded the current study.

No relevant conflicts of interest exist.

The materials and laboratory technologist time required for this study were funded by a research grant awarded to the Johns Hopkins University School of Medicine by Sienna Cancer Diagnostics.

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D.B.A. and R.S. contributed equally.

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