Abstract
Objective: Image-guided fine needle aspiration cytology with conventional smear (CS) preparation offers onsite cellular adequacy evaluation; however, it still provides false negatives due to faulty smear preparations. Liquid-based cytology (LBC) can be advantageous in these scenarios. Hence, with an aim to investigate utility of LBC in these samples, we carried out the above study with objectives to find diagnostic accuracy of LBC and agreement of LBC with CS methods in guided aspiration samples from intra-abdominal masses. Methods: A prospective observational study, of 113 patients with clinical or radiological diagnosis of intra-abdominal masses, was carried out. SurePath BD™ was used for LBC smear preparation, and the standard protocol was used for CS preparation. Results: LBC alone was diagnostic in 80.8% of the cases, and CS alone was diagnostic in 71.2% cases (agreement was 83.7%, p = 0.03). Cellular morphology was better preserved in LBC; however, interpretation was easier in CS. Conclusion: CS may be complimented with LBC sample collection method to enhance the sensitivity of intra-abdominal FNA.
References
1.
Kedar
RP
, Patel
VH
, Merchant
SA
, Aggarwal
V
, Pandit
AA
. Ultrasound guided aspiration cytology: a valuable diagnostic aid
. J Postgrad Med
. 1991
;37
:84
–7
.2.
Ciatto
S
, Catarzi
S
, Morrone
D
, Turco
MRD
. Fine-needle aspiration cytology of nonpalpable breast lesions: US versus stereotaxic guidance
. Radiology
. 1993
;188
(1
):195
–8
.3.
Hoda
RS
. Non-gynecologic cytology on liquid-based preparations: a morphologic review of facts and artifacts
. Diagn Cytopathol
. 2007
;35
(10
):621
–34
. .4.
Bandoh
N
, Goto
T
, Akahane
T
, Ohnuki
N
, Yamaguchi
T
, Kamada
H
, Diagnostic value of liquid-based cytology with fine needle aspiration specimens for cervical lymphadenopathy
. Diagn Cytopathol
. 2016 Sep
;44
(3
):169
–76
.5.
Elsheikh
TM
, Kirkpatrick
JL
, Wu
HH
. Comparison of ThinPrep and cytospin preparations in the evaluation of exfoliative cytology specimens
. Cancer
. 2006
;108
(3
):144
–9
. .6.
Agarwal
P
, Yadav
S
, Kumar
A
, Goel
MM
. Liquid-based versus conventional cytology in solid pediatric neoplasm: comparison of their diagnostic and morphological spectra
. J Cytol
. 2016
;33
(4
):199
–204
. .7.
Singh
G
, Agarwal
P
, Goel
MM
, Kumar
M
, Singh
DP
. Conventional versus liquid based cytology in fine needle aspirates of lung and mediastinal masses
. J Pulmonary Res Med
. 2017
;07
(02
):400
.8.
Glaxon
JA
, Saldanha
P
, Vasudevan
S
. Utility of usg-guided fine needle aspiration cytology in the diagnosis of intra-abdominal masses
. Ann Pathol Lab Med
. 2018
;5
:5
.9.
Likhar
KS
, Sakhuja
S
, Sawke
N
, Likhar
SK
, Mishra
MK
. Role of FNAC in diagnosis of intra-abdominal lump and its histological correlation
. Biomed Pharmacol J
. 2009
;2
:2
.10.
Reddy
S
, Andola
SK
. Fine needle aspiration cytology of intra-abdominal lesions
. J Clin Diagn Res
. 2011
;5
(3
):551
–8
.11.
Sumana
BS
, Muniyappa
B
. Ultrasonography guided fine needle aspiration cytology with preparation of cell blocks in the diagnosis of intra abdominal masses
. J Clin Diagn Res
. 2015
;9
(12
):EC08
–12
. .12.
Gangopadhyay
M
, Bhattacharyya
NK
, Ray
S
, Chakrabarty
S
, Pandit
N
. Guided fine needle aspiration cytology of retroperitoneal masses: our experience
. J Cytol
. 2011
;28
(1
):20
. .13.
Kodandaswamy
C
, Arathi
C
. Value of ultrasonography-guided fine needle aspiration cytology in the investigative sequence of hepatic lesions with an emphasis on hepatocellular carcinoma
. J Cytol
. 2011
;28
(4
):178
.14.
Mangal
N
, Sharma
VK
, Verma
N
, Agarwal
AK
, Sharma
SP
, Aneja
S
. Ultrasound guided fine needle aspiration cytology in the diagnosis of retroperitoneal masses: a study of 85 cases
. J Cytol
. 2009
;26
(3
):97
. .15.
Gibb
RK
, Martens
MG
. The impact of liquid-based cytology in decreasing the incidence of cervical cancer
. Rev ObstetGynecol
. 2011
;4
:S2
–S11
.16.
Godwin
A
, Sina
I
, Julius
B
, Daniel
O
, Ahmad
M
. Liquid based cytology of cell remnants in needles used for breast fine needle aspiration
. J Cytol
. 2010
;01
(04
):192
–4
. .17.
Gabriel
C
, Achten
R
, Drijkoningen
M
. Use of liquid-based cytology in serous fluids: a comparison with conventional cytopreparatory techniques
. Acta Cytol
. 2004
;48
(6
):825
–35
. .18.
Agarwal
G
, Garg
G
, Agarwal
P
, Goel
MM
. Liquid based cytology versus conventional cytology in fine needle aspirates of breast masses
. Ind J Res.
2019
;8
:533
.19.
Ryu
HS
, Park
IA
, Park
SY
, Jung
YY
, Park
SH
, Shin
HC
. A pilot study evaluating liquid-based fine needle aspiration cytology of breast lesions: a cytomorphological comparison of SurePath® liquid-based preparations and conventional smears
. Acta Cytol
. 2013
;57
(4
):391
–9
.20.
Agarwal
P
, Raghuvanshi
S
, Yadav
S
, Bhalla
S
, Mehrotra
R
, Gupta
S
, Role of endoscopic retrograde cholangiopancreatography guided brush cytology in evaluation of malignant biliary tract strictures: experience ofa tertiary care teaching centre in Northern India
. J Clin Diagn Res
. 2018
;12
:EC14
–EC18
.21.
Rautela
A
. Role of liquid based cytology versus conventional cytology in FNAC of abdominal masses
. Clin Exp Pathol
. 2007
.22.
Tripathy
K
, Misra
A
, Ghosh
K
. Efficacy of liquid-based cytology versus conventional smears in FNA samples
. J Cytol
. 2015 Jan
;32
(1
):17
–20
.23.
Chang
H
, Lee
E
, Lee
H
, Choi
J
, Kim
A
, Kim
BH
. Comparison of diagnostic values of thyroid aspiration samples using liquid-based preparation and conventional smear: one-year experience in a single institution
. Apmis
. 2012
;121
(2
):139
–45
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2022
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