Circulating Tumor DNA: A Step into the Future of Cancer Management

2.

Leon

SA

,

Shapiro

B

,

Sklaroff

DM

,

Yaros

MJ

.

Free DNA in the serum of cancer patients and the effect of therapy

.

Cancer Res

.

1977

Mar

;

37

(

3

):

646

–

50

.

[PubMed]

0008-5472

https://doi.org/10.1126/scitranslmed.3004742

3.

Leary

RJ

,

Sausen

M

,

Kinde

I

,

Papadopoulos

N

,

Carpten

JD

,

Craig

D

,

O’Shaughnessy

J

,

Kinzler

KW

,

Parmigiani

G

,

Vogelstein

B

,

Diaz

LA

,

Velculescu

VE

:

Detection of Chromosomal Alterations in the Circulation of Cancer Patients with Whole-Genome Sequencing.

Science translational medicine

2012

;4:162ra154-162ra154.

https://doi.org/10.1016/j.lungcan.2016.01.023

4.

Imamura

F

,

Uchida

J

,

Kukita

Y

,

Kumagai

T

,

Nishino

K

,

Inoue

T

, et al

Monitoring of treatment responses and clonal evolution of tumor cells by circulating tumor DNA of heterogeneous mutant EGFR genes in lung cancer

.

Lung Cancer

.

2016

Apr

;

94

:

68

–

73

.

[PubMed]

0169-5002

https://doi.org/10.1086/302205

5.

Lo

YM

,

Zhang

J

,

Leung

TN

,

Lau

TK

,

Chang

AM

,

Hjelm

NM

.

Rapid clearance of fetal DNA from maternal plasma

.

Am J Hum Genet

.

1999

Jan

;

64

(

1

):

218

–

24

.

[PubMed]

0002-9297

https://doi.org/10.1007/s10549-014-2946-2

6.

Madhavan

D

,

Wallwiener

M

,

Bents

K

,

Zucknick

M

,

Nees

J

,

Schott

S

, et al

Plasma DNA integrity as a biomarker for primary and metastatic breast cancer and potential marker for early diagnosis

.

Breast Cancer Res Treat

.

2014

Jul

;

146

(

1

):

163

–

74

.

[PubMed]

0167-6806

7.

Sozzi

G

,

Conte

D

,

Mariani

L

,

Lo Vullo

S

,

Roz

L

,

Lombardo

C

, et al

Analysis of circulating tumor DNA in plasma at diagnosis and during follow-up of lung cancer patients

.

Cancer Res

.

2001

Jun

;

61

(

12

):

4675

–

8

.

[PubMed]

0008-5472

8.

Bettegowda

C

,

Sausen

M

,

Leary

RJ

,

Kinde

I

,

Wang

Y

,

Agrawal

N

, et al

Shih l M, Oba-Shinjo SM, Siena S, Theodorescu D, Tie J, Harkins TT, Veronese S, Wang TL, Weingart JD, Wolfgang CL, Wood LD, Xing D, Hruban RH, Wu J, Allen PJ, Schmidt CM, Choti MA, Velculescu VE, Kinzler KW, Vogelstein B, Papadopoulos N, Diaz LA, Jr.: detection of circulating tumor DNA in early- and late-stage human malignancies

.

Sci Transl Med

.

2014

;

6

:

224ra224

.1946-6234

https://doi.org/10.1007/978-1-4939-3360-0_1

9.

Thierry

AR

.

A Targeted Q-PCR-Based Method for Point Mutation Testing by Analyzing Circulating DNA for Cancer Management Care

.

Methods Mol Biol

.

2016

;

1392

:

1

–

16

.

[PubMed]

1064-3745

https://doi.org/10.1073/pnas.0507904102

10.

Diehl

F

,

Li

M

,

Dressman

D

,

He

Y

,

Shen

D

,

Szabo

S

, et al

Detection and quantification of mutations in the plasma of patients with colorectal tumors

.

Proc Natl Acad Sci USA

.

2005

Nov

;

102

(

45

):

16368

–

73

.

[PubMed]

0027-8424

https://doi.org/10.1073/pnas.1500076112

11.

Jiang

P

,

Chan

CW

,

Chan

KC

,

Cheng

SH

,

Wong

J

,

Wong

VW

, et al

Lengthening and shortening of plasma DNA in hepatocellular carcinoma patients

.

Proc Natl Acad Sci USA

.

2015

Mar

;

112

(

11

):

E1317

–

25

.

[PubMed]

0027-8424

12.

Jahr

S

,

Hentze

H

,

Englisch

S

,

Hardt

D

,

Fackelmayer

FO

,

Hesch

RD

, et al

DNA fragments in the blood plasma of cancer patients: quantitations and evidence for their origin from apoptotic and necrotic cells

.

Cancer Res

.

2001

Feb

;

61

(

4

):

1659

–

65

.

[PubMed]

0008-5472

https://doi.org/10.1371/journal.pone.0023418

13.

Mouliere

F

,

Robert

B

,

Arnau Peyrotte

E

,

Del Rio

M

,

Ychou

M

,

Molina

F

, et al

High fragmentation characterizes tumour-derived circulating DNA

.

PLoS One

.

2011

;

6

(

9

):

e23418

.

[PubMed]

1932-6203

https://doi.org/10.1016/j.cell.2015.11.050

14.

Snyder

MW

,

Kircher

M

,

Hill

AJ

,

Daza

RM

,

Shendure

J

.

Cell-free DNA Comprises an In Vivo Nucleosome Footprint that Informs Its Tissues-Of-Origin

.

Cell

.

2016

Jan

;

164

(

1-2

):

57

–

68

.

[PubMed]

0092-8674

15.

Soo

J

,

Kurtz

DM

,

Jin

M

,

Scherer

F

,

Schroers-Martin

JG

,

Esfahani

MS

, et al

Comparison of Circulating Tumor DNA Recovery from Plasma and Serum

.

Blood

.

2017

;

130

:

2756

–

2756

.0006-4971

16.

Park

JL

,

Kim

HJ

,

Choi

BY

,

Lee

HC

,

Jang

HR

,

Song

KS

, et al

Quantitative analysis of cell-free DNA in the plasma of gastric cancer patients

.

Oncol Lett

.

2012

Apr

;

3

(

4

):

921

–

6

.

[PubMed]

1792-1074

17.

Molina-Vila

MA

,

de-Las-Casas

CM

,

Bertran-Alamillo

J

,

Jordana-Ariza

N

,

González-Cao

M

,

Rosell

R

.

cfDNA analysis from blood in melanoma

.

Ann Transl Med

.

2015

Nov

;

3

(

20

):

309

.

[PubMed]

2305-5839

https://doi.org/10.1002/cam4.1184

18.

van Ginkel

JH

,

van den Broek

DA

,

van Kuik

J

,

Linders

D

,

de Weger

R

,

Willems

SM

, et al

Preanalytical blood sample workup for cell-free DNA analysis using Droplet Digital PCR for future molecular cancer diagnostics

.

Cancer Med

.

2017

Oct

;

6

(

10

):

2297

–

307

.

[PubMed]

2045-7634

19.

Botezatu

I

,

Serdyuk

O

,

Potapova

G

,

Shelepov

V

,

Alechina

R

,

Molyaka

Y

, et al

Genetic analysis of DNA excreted in urine: a new approach for detecting specific genomic DNA sequences from cells dying in an organism

.

Clin Chem

.

2000

Aug

;

46

(

8 Pt 1

):

1078

–

84

.

[PubMed]

0009-9147

https://doi.org/10.1016/S1525-1578(10)60497-7

20.

Su

YH

,

Wang

M

,

Brenner

DE

,

Ng

A

,

Melkonyan

H

,

Umansky

S

, et al

Human urine contains small, 150 to 250 nucleotide-sized, soluble DNA derived from the circulation and may be useful in the detection of colorectal cancer

.

J Mol Diagn

.

2004

May

;

6

(

2

):

101

–

7

.

[PubMed]

1525-1578

https://doi.org/10.1373/clinchem.2014.235457

21.

Pan

W

,

Gu

W

,

Nagpal

S

,

Gephart

MH

,

Quake

SR

.

Brain tumor mutations detected in cerebral spinal fluid

.

Clin Chem

.

2015

Mar

;

61

(

3

):

514

–

22

.

[PubMed]

0009-9147

https://doi.org/10.1038/ejhg.2015.281

22.

Togneri

FS

,

Ward

DG

,

Foster

JM

,

Devall

AJ

,

Wojtowicz

P

,

Alyas

S

, et al

Genomic complexity of urothelial bladder cancer revealed in urinary cfDNA

.

Eur J Hum Genet

.

2016

Aug

;

24

(

8

):

1167

–

74

.

[PubMed]

1018-4813

https://doi.org/10.18632/oncotarget.9027

23.

Xia

Y

,

Huang

CC

,

Dittmar

R

,

Du

M

,

Wang

Y

,

Liu

H

, et al

Copy number variations in urine cell free DNA as biomarkers in advanced prostate cancer

.

Oncotarget

.

2016

Jun

;

7

(

24

):

35818

–

31

.

[PubMed]

1949-2553

https://doi.org/10.18632/oncotarget.20004

24.

Perdomo

S

,

Avogbe

PH

,

Foll

M

,

Abedi-Ardekani

B

,

Facciolla

VL

,

Anantharaman

D

, et al

Circulating tumor DNA detection in head and neck cancer: evaluation of two different detection approaches

.

Oncotarget

.

2017

Aug

;

8

(

42

):

72621

–

32

.

[PubMed]

1949-2553

https://doi.org/10.21037/tcr.2017.02.23

25.

Lianidou

E

,

Alix-Panabières

C

.

Early detection of lung cancer based on DNA methylation analysis in sputum and plasma

.

Transl Cancer Res

.

2017

;

6

S1

:

S51

–

3

.

2218-676X

https://doi.org/10.1038/ncomms9839

26.

De Mattos-Arruda

L

,

Mayor

R

,

Ng

CK

,

Weigelt

B

,

Martínez-Ricarte

F

,

Torrejon

D

, et al

Cerebrospinal fluid-derived circulating tumour DNA better represents the genomic alterations of brain tumours than plasma

.

Nat Commun

.

2015

Nov

;

6

(

1

):

8839

.

[PubMed]

2041-1723

https://doi.org/10.1097/PPO.0000000000000308

27.

Ramalingam

N

,

Jeffrey

SS

.

Future of Liquid Biopsies With Growing Technological and Bioinformatics Studies: Opportunities and Challenges in Discovering Tumor Heterogeneity With Single-Cell Level Analysis

.

Cancer J

.

2018

Mar/Apr

;

24

(

2

):

104

–

8

.

[PubMed]

1528-9117

https://doi.org/10.1186/s12916-016-0667-6

28.

Cohen

PA

,

Flowers

N

,

Tong

S

,

Hannan

N

,

Pertile

MD

,

Hui

L

.

Abnormal plasma DNA profiles in early ovarian cancer using a non-invasive prenatal testing platform: implications for cancer screening

.

BMC Med

.

2016

Aug

;

14

(

1

):

126

.

[PubMed]

1741-7015

https://doi.org/10.1007/s12253-018-0382-z

29.

Bartak

BK

,

Kalmar

A

,

Galamb

O

,

Wichmann

B

,

Nagy

ZB

,

Tulassay

Z

,

Dank

M

,

Igaz

P

,

Molnar

B

:

Blood Collection and Cell-Free DNA Isolation Methods Influence the Sensitivity of Liquid Biopsy Analysis for Colorectal Cancer Detection.

Pathology oncology research : POR

2018

https://doi.org/10.1038/s41598-018-25810-0

30.

Markus

H

,

Contente-Cuomo

T

,

Farooq

M

,

Liang

WS

,

Borad

MJ

,

Sivakumar

S

, et al

Evaluation of pre-analytical factors affecting plasma DNA analysis

.

Sci Rep

.

2018

May

;

8

(

1

):

7375

.

[PubMed]

2045-2322

https://doi.org/10.1016/bs.acc.2015.03.002

31.

Zonta

E

,

Nizard

P

,

Taly

V

.

Assessment of DNA Integrity, Applications for Cancer Research

.

Adv Clin Chem

.

2015

;

70

:

197

–

246

.

[PubMed]

0065-2423

https://doi.org/10.1016/j.gpb.2016.12.004

32.

Han

X

,

Wang

J

,

Sun

Y

.

Circulating Tumor DNA as Biomarkers for Cancer Detection

.

Genomics Proteomics Bioinformatics

.

2017

Apr

;

15

(

2

):

59

–

72

.

[PubMed]

1672-0229

https://doi.org/10.1373/clinchem.2015.238717

33.

Guttery

DS

,

Page

K

,

Hills

A

,

Woodley

L

,

Marchese

SD

,

Rghebi

B

, et al

Noninvasive detection of activating estrogen receptor 1 (ESR1) mutations in estrogen receptor-positive metastatic breast cancer

.

Clin Chem

.

2015

Jul

;

61

(

7

):

974

–

82

.

[PubMed]

0009-9147

https://doi.org/10.1371/journal.pone.0194630

34.

Gale

D

,

Lawson

AR

,

Howarth

K

,

Madi

M

,

Durham

B

,

Smalley

S

, et al

Development of a highly sensitive liquid biopsy platform to detect clinically-relevant cancer mutations at low allele fractions in cell-free DNA

.

PLoS One

.

2018

Mar

;

13

(

3

):

e0194630

.

[PubMed]

1932-6203

https://doi.org/10.18632/oncotarget.11717

35.

Bennett

CW

,

Berchem

G

,

Kim

YJ

,

El-Khoury

V

.

Cell-free DNA and next-generation sequencing in the service of personalized medicine for lung cancer

.

Oncotarget

.

2016

Oct

;

7

(

43

):

71013

–

35

.

[PubMed]

1949-2553

https://doi.org/10.1038/s41598-018-20282-8

36.

Christensen

E

,

Nordentoft

I

,

Vang

S

,

Birkenkamp-Demtröder

K

,

Jensen

JB

,

Agerbæk

M

, et al

Optimized targeted sequencing of cell-free plasma DNA from bladder cancer patients

.

Sci Rep

.

2018

Jan

;

8

(

1

):

1917

.

[PubMed]

2045-2322

https://doi.org/10.1038/srep35451

37.

Dobnik

D

,

Štebih

D

,

Blejec

A

,

Morisset

D

,

Žel

J

.

Multiplex quantification of four DNA targets in one reaction with Bio-Rad droplet digital PCR system for GMO detection

.

Sci Rep

.

2016

Oct

;

6

(

1

):

35451

.

[PubMed]

2045-2322

https://doi.org/10.1186/s12916-017-0840-6

38.

Perakis

S

,

Speicher

MR

.

Emerging concepts in liquid biopsies

.

BMC Med

.

2017

Apr

;

15

(

1

):

75

.

[PubMed]

1741-7015

https://doi.org/10.1001/jamaoncol.2016.0173

39.

Sacher

AG

,

Paweletz

C

,

Dahlberg

SE

,

Alden

RS

,

O’Connell

A

,

Feeney

N

, et al

Prospective Validation of Rapid Plasma Genotyping for the Detection of EGFR and KRAS Mutations in Advanced Lung Cancer

.

JAMA Oncol

.

2016

Aug

;

2

(

8

):

1014

–

22

.

[PubMed]

2374-2437

https://doi.org/10.1007/978-1-4939-7778-9_4

40.

Whale

AS

,

Fernandez-Gonzalez

A

,

Gutteridge

A

,

Devonshire

AS

.

Control Materials and Digital PCR Methods for Evaluation of Circulating Cell-Free DNA Extractions from Plasma

.

Methods Mol Biol

.

2018

;

1768

:

45

–

65

.

[PubMed]

1064-3745

https://doi.org/10.21037/atm.2017.01.32

41.

Kwapisz

D

.

The first liquid biopsy test approved. Is it a new era of mutation testing for non-small cell lung cancer?

Ann Transl Med

.

2017

Feb

;

5

(

3

):

46

.

[PubMed]

2305-5839

https://doi.org/10.1200/JCO.2017.76.8671

42.

Qiagen: QIAGEN announces first-ever regulatory registration of a lung cancer companion diagnostic based on liquid biopsies.

2015

43.

Merker

JD

,

Oxnard

GR

,

Compton

C

,

Diehn

M

,

Hurley

P

,

Lazar

AJ

, et al

Circulating Tumor DNA Analysis in Patients With Cancer: American Society of Clinical Oncology and College of American Pathologists Joint Review

.

J Clin Oncol

.

2018

Jun

;

36

(

16

):

1631

–

41

.

[PubMed]

0732-183X

https://doi.org/10.1373/clinchem.2014.222679

44.

Heitzer

E

,

Ulz

P

,

Geigl

JB

.

Circulating tumor DNA as a liquid biopsy for cancer

.

Clin Chem

.

2015

Jan

;

61

(

1

):

112

–

23

.

[PubMed]

0009-9147

https://doi.org/10.2147/LCTT.S147841

45.

Veldore

VH

,

Choughule

A

,

Routhu

T

,

Mandloi

N

,

Noronha

V

,

Joshi

A

, et al

Validation of liquid biopsy: plasma cell-free DNA testing in clinical management of advanced non-small cell lung cancer

.

Lung Cancer (Auckl)

.

2018

Jan

;

9

:

1

–

11

.

[PubMed]

1179-2728

https://doi.org/10.1200/JCO.2003.02.006

46.

Sozzi

G

,

Conte

D

,

Leon

M

,

Ciricione

R

,

Roz

L

,

Ratcliffe

C

, et al

Quantification of free circulating DNA as a diagnostic marker in lung cancer

.

J Clin Oncol

.

2003

Nov

;

21

(

21

):

3902

–

8

.

[PubMed]

0732-183X

https://doi.org/10.3892/ol.2015.3771

47.

Shao

X

,

He

Y

,

Ji

M

,

Chen

X

,

Qi

J

,

Shi

W

, et al

Quantitative analysis of cell-free DNA in ovarian cancer

.

Oncol Lett

.

2015

Dec

;

10

(

6

):

3478

–

82

.

[PubMed]

1792-1074

https://doi.org/10.3389/fmed.2018.00009

48.

Hench

IB

,

Hench

J

,

Tolnay

M

.

Liquid Biopsy in Clinical Management of Breast, Lung, and Colorectal Cancer

.

Front Med (Lausanne)

.

2018

Jan

;

5

:

9

.

[PubMed]

2296-858X

https://doi.org/10.1177/0961203311399606

49.

Bartoloni

E

,

Ludovini

V

,

Alunno

A

,

Pistola

L

,

Bistoni

O

,

Crinò

L

, et al

Increased levels of circulating DNA in patients with systemic autoimmune diseases: A possible marker of disease activity in Sjögren’s syndrome

.

Lupus

.

2011

Aug

;

20

(

9

):

928

–

35

.

[PubMed]

0961-2033

https://doi.org/10.1016/j.tranon.2017.12.010

50.

Klein-Scory

S

,

Maslova

M

,

Pohl

M

,

Eilert-Micus

C

,

Schroers

R

,

Schmiegel

W

, et al

Significance of Liquid Biopsy for Monitoring and Therapy Decision of Colorectal Cancer

.

Transl Oncol

.

2018

Apr

;

11

(

2

):

213

–

20

.

[PubMed]

1936-5233

51.

Provencio

M

,

Torrente

M

,

Calvo

V

,

Pérez-Callejo

D

,

Gutiérrez

L

,

Franco

F

, et al

Prognostic value of quantitative ctDNA levels in non small cell lung cancer patients

.

Oncotarget

.

2017

Nov

;

9

(

1

):

488

–

94

.

[PubMed]

1949-2553

https://doi.org/10.1158/1078-0432.CCR-15-0943

52.

Chu

D

,

Paoletti

C

,

Gersch

C

,

VanDenBerg

DA

,

Zabransky

DJ

,

Cochran

RL

, et al

ESR1 Mutations in Circulating Plasma Tumor DNA from Metastatic Breast Cancer Patients

.

Clin Cancer Res

.

2016

Feb

;

22

(

4

):

993

–

9

.

[PubMed]

1078-0432

https://doi.org/10.1126/scitranslmed.aac7551

53.

Schiavon

G

,

Hrebien

S

,

Garcia-Murillas

I

,

Cutts

RJ

,

Pearson

A

,

Tarazona

N

, et al

Analysis of ESR1 mutation in circulating tumor DNA demonstrates evolution during therapy for metastatic breast cancer

.

Sci Transl Med

.

2015

Nov

;

7

(

313

):

313ra182

.

[PubMed]

1946-6234

https://doi.org/10.1158/1078-0432.CCR-17-0510

54.

Schøler

LV

,

Reinert

T

,

Ørntoft

MW

,

Kassentoft

CG

,

Árnadóttir

SS

,

Vang

S

, et al

Clinical Implications of Monitoring Circulating Tumor DNA in Patients with Colorectal Cancer

.

Clin Cancer Res

.

2017

Sep

;

23

(

18

):

5437

–

45

.

[PubMed]

1078-0432

https://doi.org/10.1016/j.clcc.2017.10.017

55.

Germano

G

,

Mauri

G

,

Siravegna

G

,

Dive

C

,

Pierce

J

,

Di Nicolantonio

F

, et al

Parallel Evaluation of Circulating Tumor DNA and Circulating Tumor Cells in Metastatic Colorectal Cancer

.

Clin Colorectal Cancer

.

2018

Mar

;

17

(

1

):

80

–

3

.

[PubMed]

1533-0028

https://doi.org/10.1002/cam4.980

56.

Hu

W

,

Yang

Y

,

Zhang

L

,

Yin

J

,

Huang

J

,

Huang

L

, et al

Post surgery circulating free tumor DNA is a predictive biomarker for relapse of lung cancer

.

Cancer Med

.

2017

May

;

6

(

5

):

962

–

74

.

[PubMed]

2045-7634

https://doi.org/10.1016/j.cllc.2017.02.005

57.

Heitzer

E

,

Perakis

S

,

Geigl

JB

,

Speicher

MR

: The potential of liquid biopsies for the early detection of cancer. npj Precision Oncology

2017

;1:36.

58.

Remon

J

,

Menis

J

,

Hasan

B

,

Peric

A

,

De Maio

E

,

Novello

S

, et al

The APPLE Trial: Feasibility and Activity of AZD9291 (Osimertinib) Treatment on Positive PLasma T790M in EGFR-mutant NSCLC Patients. EORTC 1613

.

Clin Lung Cancer

.

2017

Sep

;

18

(

5

):

583

–

8

.

[PubMed]

1525-7304

https://doi.org/10.1016/j.lungcan.2017.04.004

59.

Knebel

FH

,

Bettoni

F

,

Shimada

AK

,

Cruz

M

,

Alessi

JV

,

Negrão

MV

, et al

Sequential liquid biopsies reveal dynamic alterations of EGFR driver mutations and indicate EGFR amplification as a new mechanism of resistance to osimertinib in NSCLC

.

Lung Cancer

.

2017

Jun

;

108

:

238

–

41

.

[PubMed]

0169-5002

https://doi.org/10.1016/j.amjms.2017.09.009

60.

Sun

Q

,

Liu

Y

,

Liu

B

,

Liu

Y

.

Use of Liquid Biopsy in Monitoring Colorectal Cancer Progression Shows Strong Clinical Correlation

.

Am J Med Sci

.

2018

Mar

;

355

(

3

):

220

–

7

.

[PubMed]

0002-9629

https://doi.org/10.1186/s13059-017-1191-5

61.

Kang

S

,

Li

Q

,

Chen

Q

,

Zhou

Y

,

Park

S

,

Lee

G

, et al

CancerLocator: non-invasive cancer diagnosis and tissue-of-origin prediction using methylation profiles of cell-free DNA

.

Genome Biol

.

2017

Mar

;

18

(

1

):

53

.

[PubMed]

1474-7596

https://doi.org/10.1186/s12920-015-0130-0

62.

Marquard

AM

,

Birkbak

NJ

,

Thomas

CE

,

Favero

F

,

Krzystanek

M

,

Lefebvre

C

, et al

TumorTracer: a method to identify the tissue of origin from the somatic mutations of a tumor specimen

.

BMC Med Genomics

.

2015

Oct

;

8

(

1

):

58

.

[PubMed]

1755-8794

https://doi.org/10.1126/science.aar3247

63.

Cohen

JD

,

Li

L

,

Wang

Y

,

Thoburn

C

,

Afsari

B

,

Danilova

L

, et al

Detection and localization of surgically resectable cancers with a multi-analyte blood test

.

Science

.

2018

Feb

;

359

(

6378

):

926

–

30

.

[PubMed]

0036-8075

https://doi.org/10.1007/s00428-012-1354-4

64.

van Krieken

JH

,

Normanno

N

,

Blackhall

F

,

Boone

E

,

Botti

G

,

Carneiro

F

, et al

Guideline on the requirements of external quality assessment programs in molecular pathology

.

Virchows Arch

.

2013

Jan

;

462

(

1

):

27

–

37

.

[PubMed]

0945-6317

https://doi.org/10.1200/JCO.2016.34.15_suppl.e23175

65.

Shen

W

,

Zhao

GQ

,

Liang

Z

,

Yellapantula

V

,

Sun

S

,

Tang

LF

, et al

Retrospective analysis of circulating tumor DNA of archived plasma samples

.

J Clin Oncol

.

2016

;

34

15_suppl

:

e23175

–

23175

.

0732-183X

66.

Chan

BA

,

Hughes

BG

.

Targeted therapy for non-small cell lung cancer: current standards and the promise of the future

.

Transl Lung Cancer Res

.

2015

Feb

;

4

(

1

):

36

–

54

.

[PubMed]

2218-6751

https://doi.org/10.1016/j.lungcan.2016.04.026

67.

Sorber

L

,

Zwaenepoel

K

,

Deschoolmeester

V

,

Van Schil

PE

,

Van Meerbeeck

J

,

Lardon

F

, et al

Circulating cell-free nucleic acids and platelets as a liquid biopsy in the provision of personalized therapy for lung cancer patients

.

Lung Cancer

.

2017

May

;

107

:

100

–

7

.

[PubMed]

0169-5002

https://doi.org/10.21037/atm.2017.04.12

68.

Köhn

L

,

Johansson

M

,

Grankvist

K

,

Nilsson

J

.

Liquid biopsies in lung cancer-time to implement research technologies in routine care?

Ann Transl Med

.

2017

Jul

;

5

(

13

):

278

.

[PubMed]

2305-5839

https://doi.org/10.21037/tlcr.2016.10.07

69.

Pérez-Callejo

D

,

Romero

A

,

Provencio

M

,

Torrente

M

.

Liquid biopsy based biomarkers in non-small cell lung cancer for diagnosis and treatment monitoring

.

Transl Lung Cancer Res

.

2016

Oct

;

5

(

5

):

455

–

65

.

[PubMed]

2218-6751

https://doi.org/10.1016/j.tig.2015.06.001

70.

Cai

X

,

Janku

F

,

Zhan

Q

,

Fan

JB

.

Accessing Genetic Information with Liquid Biopsies

.

Trends Genet

.

2015

Oct

;

31

(

10

):

564

–

75

.

[PubMed]

0168-9525

https://doi.org/10.1038/s41598-017-18642-x

71.

Qiagen: therascreen® EGFR RGQ PCR Kit Instructions for Use (Handbook).

2013

72.

Kim

SS

,

Choi

HJ

,

Kim

JJ

,

Kim

MS

,

Lee

IS

,

Byun

B

, et al

Droplet digital PCR-based EGFR mutation detection with an internal quality control index to determine the quality of DNA

.

Sci Rep

.

2018

Jan

;

8

(

1

):

543

.

[PubMed]

2045-2322

https://doi.org/10.1093/annonc/mdu288

73.

Rothé

F

,

Laes

JF

,

Lambrechts

D

,

Smeets

D

,

Vincent

D

,

Maetens

M

, et al

Plasma circulating tumor DNA as an alternative to metastatic biopsies for mutational analysis in breast cancer

.

Ann Oncol

.

2014

Oct

;

25

(

10

):

1959

–

65

.

[PubMed]

0923-7534

https://doi.org/10.1126/scitranslmed.3003726

74.

Forshew

T

,

Murtaza

M

,

Parkinson

C

,

Gale

D

,

Tsui

DW

,

Kaper

F

, et al

Noninvasive identification and monitoring of cancer mutations by targeted deep sequencing of plasma DNA

.

Sci Transl Med

.

2012

May

;

4

(

136

):

136ra68

.

[PubMed]

1946-6234

https://doi.org/10.1038/nm.3519

75.

Newman

AM

,

Bratman

SV

,

To

J

,

Wynne

JF

,

Eclov

NC

,

Modlin

LA

, et al

An ultrasensitive method for quantitating circulating tumor DNA with broad patient coverage

.

Nat Med

.

2014

May

;

20

(

5

):

548

–

54

.

[PubMed]

1078-8956

https://doi.org/10.1073/pnas.1105422108

76.

Kinde

I

,

Wu

J

,

Papadopoulos

N

,

Kinzler

KW

,

Vogelstein

B

.

Detection and quantification of rare mutations with massively parallel sequencing

.

Proc Natl Acad Sci USA

.

2011

Jun

;

108

(

23

):

9530

–

5

.

[PubMed]

0027-8424

https://doi.org/10.1038/nrclinonc.2017.14

77.

Siravegna

G

,

Marsoni

S

,

Siena

S

,

Bardelli

A

.

Integrating liquid biopsies into the management of cancer

.

Nat Rev Clin Oncol

.

2017

Sep

;

14

(

9

):

531

–

48

.

[PubMed]

1759-4774