Objectives: To estimate the frequency of human papillomavirus (HPV) infection and the genotype distribution of HPV among women with a Pap smear showing atypical squamous cells of undetermined significance (ASC-US) attending the Program for the Detection and Control of Cervical Cancer in Bogotá, Colombia. Study Design: Cervical samples from 200 women with an ASC-US Pap smear were analyzed for the presence of HPV DNA and genotype distribution using a commercial molecular technique (Linear Array®; Roche Molecular Systems, USA). Results: HPV infection was found in 140 women (70%). High-risk HPV types were present in 46.4% of the samples; 16.4% showed a low-risk HPV type, and 37.1% showed both. Of the positive samples, 42.9% were infected with a single viral genotype, whereas 57.1% exhibited multiple HPV infections. The most common HPV genotypes were HPV 16, 53, and 52 with a prevalence of 26.4, 16.4, and 13.6%, respectively. Conclusion: The epidemiological characterization of HPV infections described in this study might guide actions for epidemiological surveillance to strengthen the program in Bogotá and to develop appropriate HPV vaccination programs.

1.
Farfán-Vargas YA, García-Robayo DA, Arias Murillo Y, Morales OL, Isaza M, Aristizábal Gutiérrez FA: Genotipificación del virus de papiloma humano en mujeres con hallazgo citológico de lesión escamosa intraepitelial de bajo grado (LSIL) o de significado indeterminado (ASC-US) en Bogotá, Colombia. Rev Colomb Cienc Quim Farm 2010;39:42-54.
2.
Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D, Bray F: Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer 2015;136:E359-E386.
[PubMed]
3.
Bernard W, Stewart, Wild C: World Cancer Report 2014. Lyon, International Agency for Research on Cancer, World Health Organization, 2014.
4.
Soto-De Leon S, Camargo M, Sanchez R, Munoz M, Perez-Prados A, Purroy A, et al: Distribution patterns of infection with multiple types of human papillomaviruses and their association with risk factors. PLoS One 2011;6:e14705.
[PubMed]
5.
Chao TK, Ke FY, Liao YP, Wang HC, Yu CP, Lai HC: Triage of cervical cytological diagnoses of atypical squamous cells by DNA methylation of paired boxed gene 1 (PAX1). Diagn Cytopathol 2013;41:41-46.
[PubMed]
6.
Zhang S-K, Ci P-W, Velicer C, Kang L-N, Liu B, Cui J-F, et al: Comparison of HPV genotypes and viral load between different sites of genital tract: the significance for cervical cancer screening. Cancer Epidemiol 2014;38:168-173.
[PubMed]
7.
Molano M, Posso H, Weiderpass E, Van den Brule A, Ronderos M, Franceschi S, et al: Prevalence and determinants of HPV infection among Colombian women with normal cytology. Br J Cancer 2002;87:324-333.
[PubMed]
8.
Faridi R, Zahra A, Khan K, Idrees M: Oncogenic potential of Human Papillomavirus. Virol J 2011;8:269.
[PubMed]
9.
Carter JR, Ding Z, Rose BR: HPV infection and cervical disease: a review. Aust NZJ Obstet Gynaecol 2011;51:103-108.
[PubMed]
10.
Argyri E, Papaspyridakos S, Tsimplaki E, Michala L, Myriokefalitaki E, Papassideri I, et al: A cross sectional study of HPV type prevalence according to age and cytology. BMC Infect Dis 2013;13:53.
[PubMed]
11.
López A, Lizano M: Cáncer cervicouterino y el virus del papiloma humano: la historia que no termina. Cancerología 2006;1:31-55.
12.
Venezuela RF, Kiguen AX, Frutos MC, Cuffini CG: Circulation of human papillomavirus (HPV) genotypes in women from Córdoba, Argentina, with squamous intraepithelial lesions. Rev Inst Med Trop São Paulo 2012;54:11-16.
[PubMed]
13.
Nayar R, Wilbur D: The Bethesda System for Reporting Cervical Cytology: Definitions, Criteria and Explanatory Notes. Cham, Springer, 2015, pp 103-192.
14.
Wright TC, Stoler MH, Behrens CM, Apple R, Derion T, Wright TL: The ATHENA human papillomavirus study: design, methods, and baseline results. Am J Obstet Gynecol 2012;206:46.e1-46.e11.
[PubMed]
15.
Barcelos ACM, Michelin MA, Adad SJ, Murta EFC: Atypical squamous cells of undetermined significance: Bethesda classification and association with human papillomavirus. Infect Dis Obstet Gynecol 2011;2011:904674.
[PubMed]
16.
Arbyn M, Buntinx F, Van Ranst M, Paraskevaidis E, Martin-Hirsch P, Dillner J: Virologic versus cytologic triage of women with equivocal Pap smears: a meta-analysis of the accuracy to detect high-grade intraepithelial neoplasia. J Natl Cancer Inst 2004;96:280-293.
[PubMed]
17.
Delgado D, Marín JM, de Diego J, Guerra S, González B, Barrios JL, et al: Human papillomavirus (HPV) genotype distribution in women with abnormal cervical cytology in the Basque Country, Spain. Enferm Infecc Microbiol Clin 2012;30:230-235.
[PubMed]
18.
Martín P, Kilany L, García D, López-García AM, Martín-Azaña MJ, Abraira V, et al: Human papillomavirus genotype distribution in Madrid and correlation with cytological data. BMC Infect Dis 2011;11:316.
[PubMed]
19.
Shen Y, Gong J-M, Li Y-Q, Gong Y-M, Lei D-M, Cheng G-M, et al: Epidemiology and genotype distribution of human papillomavirus (HPV) in women of Henan Province, China. Clin Chim Acta 2013;415:297-301.
[PubMed]
20.
Dickson EL, Vogel RI, Geller MA, Downs LS: Cervical cytology and multiple type HPV infection: a study of 8,182 women ages 31-65. Gynecol Oncol 2014;133:405-408.
[PubMed]
21.
Núñez-Troconis J, Delgado M, González J, Mindiola R, Velásquez J, Conde B, et al: Prevalence and risk factors of human papillomavirus infection in asymptomatic women in a Venezuelan urban area. Invest Clin 2009;50:203-212.
[PubMed]
22.
Spiryda LB, Brown J, Zhang H, Burgis JT: Delaying Pap test screening in the adolescent population: an evidence-based approach. J Pediatr Adolesc Gynecol 2014;27:3-5.
[PubMed]
23.
Sijvarger C, González J, Prieto A, Messmer A, Mallimaci M, Alonio V, et al: Epidemiología de la infección cervical por virus papiloma humano en Ushuaia, Argentina. Rev Argent Microbiol 2006;38:19-24.
[PubMed]
24.
Castle PE, Gage JC, Wheeler CM, Schiffman M: The clinical meaning of a cervical intraepithelial neoplasia grade 1 biopsy. Obstet Gynecol 2011;118:1222-1229.
[PubMed]
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