Objective: Thyroid transcription factor 1 (TTF-1)/napsin A double staining is useful for the identification of primary lung adenocarcinoma. We examined its performance in pleural fluid. Study Design: Thirty-eight pleural-fluid specimens with lung adenocarcinoma were identified. Among these, 23 cases were diagnosed with a lung primary adenocarcinoma and 15 with a nonlung primary adenocarcinoma [breast (n = 13), ovarian (n = 1) and esophageal (n = 1)]. Immunohistochemistry was performed on formalin-fixed, paraffin-embedded cell blocks. Expression of TTF-1 as a brown nuclear stain and napsin A as a red cytoplasmic stain were identified as positive staining. Results: In lung adenocarcinoma, 19 out of the 23 (83%) cases were positive for the TTF-1/napsin A double stain, 1 (4%) was positive for TTF-1 only, 2 (9%) were positive for napsin A only and 1 (4%) was negative for the TTF-1/napsin A double stain. All 15 cases of nonlung primary adenocarcinoma were negative for the TTF-1/napsin A double stain. Conclusion: The sensitivity of napsin A for diagnosing lung adenocarcinoma is slightly higher than that of TTF-1.

Keywords:
Thyroid transcription factor 1, Napsin A, Adenocarcinoma, Pleural fluid
© 2012 S. Karger AG, Basel
2012
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.