Objective: The present study was undertaken to assess the therapeutic effects of topical ozonated oil on early healing of free gingival graft surgical sites. Study Design: Twenty subjects were entered into this triple-blinded, randomized, placebo-controlled clinical trial, designed to evaluate the efficacy of ozonated oil on free gingival graft surgical wounds. Subjects were assigned to either the ozone group, in which ozonated oil was applied to the surgical wound, or the control group, in which non-ozonated oil was used as a control. Patients were postoperatively evaluated by cytological analysis. Cytological analysis consisted of the keratinisation and superficial cell indices measured at baseline, after 24 h, on the 3rd, 7th, 14th and 21st day and 2, 3, 8 and 18 months postoperatively. Results: Cytological results showed that there was a significant (p < 0.001) improvement in epithelial healing by the 7th, 14th and 21st day and 2, 3 and 8 months postoperatively in the ozone group compared to the control group. Conclusion: The present study showed significant improvement in epithelial healing and gingival health after topical application of ozone-treated plant oil to gingival surgical sites.

Keywords:
Cytological techniques, Ozonated oil, Epithelial healing, Cytological analysis, Ozone therapy, Gingival wounds
1.
Witte M, Barbul A: General principles of wound healing. Surg Clin North Am 1997;77:509–528.
2.
Häkkinen L, Uitto VJ, Larjava H: Cell biology of gingival wound healing. Periodontol 2000 2000;24:127–152.
3.
Camargo P, Melnick PR, Kenney EB: The use of free gingival grafts for aesthetic purposes. Periodontol 2000 2001;27:72–96.
4.
Kozlovsky A, Artzi Z, Hirshberg A, Israeli-Tobias C, Reich L: Effect of local antimicrobial agents on excisional palatal wound healing: a clinical and histomorphometric study in rats. J Clin Periodontol 2007;34:164–171.
5.
Nooh N, Graves DT: Healing is delayed in oral compared to dermal excisional wounds. J Periodontol 2003;74:242–246.
6.
Nyman S, Lindhe J, Rosling B: Periodontal surgery in plaque-infected dentitions. J Clin Periodontol 1977;4:240–249.
7.
Thomas GW, Rael LT, Bar-Or R, et al: Mechanisms of delayed wound healing by commonly used antiseptics. J Trauma 2009;66:82–90.
8.
Huth KC, Jakob FM, Saugel B, et al: Effect of ozone on oral cells compared with established antimicrobials. Eur J Oral Sci 2006;14:435–440.
9.
Nagayoshi M, Fukuizumi T, Kitamura C, Yano J, Terashita M, Nishihara T: Efficacy of ozone on survival and permeability of oral micro-organisms. Oral Microbiol Immunol 2004;19:240–246.
10.
Ishizaki K, Sawadaishi D, Miura K, Shinriki N: Effect of ozone on plasmid DNA of Escherichia coli in situ. Water Res 1987;21:823–828.
11.
Mudd JB, Leavitt R, Ongun A, McManus T: Reaction of ozone with amino acids and proteins. Atmos Environ 1969;3:669–682.
12.
Verrazzo G, Coppola L, Luongo C, et al: Hyperbaric oxygen, oxygen-ozone therapy, and rheologic parameters of blood in patients with peripheral occlusive arterial disease. Undersea Hyperb Med 1995;22:17–22.
13.
Shiratori R, Kaneko Y, Kobayashi Y, et al: Can ozone administration activate the tissue metabolism-a study on brain metabolism during hypoxic hypoxia (in Japanese). Masui 1993;42:2–6.
14.
Oosting RS, Van Rees-Verhoef M, Verhoef J, Van Golde LM, Van Bree L: Effects of ozone on cellular ATP levels in rat and mouse alveolar macrophages. Toxicology 1991;70:195–202.
15.
Mlinek A, Smukler H, Buchner A: The use of free gingival grafts for the coverage of denuded roots. J Periodontol 1973;44:248–254.
16.
Sciubba JJ: Improving detection of precancerous and cancerous oral lesions. Computer-assisted analysis of the oral brush biopsy. U.S. Collaborative OralCDx Study Group. J Am Dent Assoc 1999;130:1445–1457.
17.
Lange DE, Bernimoulin JP: Exfoliative cytological studies in evaluation of free gingival graft healing. J Clin Periodontol 1974;1:89–96.
18.
Broughton G 2nd, Janis JE, Attinger CE: The basic science of wound healing. Plast Reconstr Surg 2006;117:12–34.
19.
Stegemann EA, Lange DE, Wegner C: Experimental studies on the standardization of oral exfoliative cytological determination procedures using the keratinisation index (in German). Dtsch Zahnärztl Z 1974;29:472–477.
20.
Bocci V, Luzzi E, Corradeschi F, Silvestri S: Studies on the biological effects of ozone. 6. Production of transforming growth factor 1 by human blood after ozone treatment. J Biol Regul Homeost Agents 1994;8:108–112.
21.
Bocci V: Oxygen-Ozone Therapy. A Critical Evaluation, ed 2. London, Kluwer, 2002.
22.
Bocci V, Paulesu L: Studies on the biological effects of ozone. 1. Induction of interferon gamma on human leucocytes. Haematologica 1990;75:510–515.
23.
Bocci V, Luzzi E, Corradeschi F, Paulesu L, Rossi R, Cardaioli E, Di Simplicio P: Studies on the biological effects of ozone. 4. Cytokine production and glutathione levels in human erythrocytes. J Biol Regul Homeost Agents 1998;12:67–75.
24.
Martinez-Sanchez G, Al-Dalain SM, Menendez S, et al: Therapeutic efficacy of ozone in patients with diabetic foot. Eur J Pharmacol 2005;523:151–161.
25.
Robson MC, Stenberg BD, Heggers JP: Wound healing alterations caused by infection. Clin Plast Surg 1990;17:485–492.
26.
Thomson P: The microbiology of wounds. J Wound Care 1998;7:477–478.
27.
Edwards R, Harding KG: Bacteria and wound healing. Curr Opin Infect Dis 2004;17:91–96.
28.
McGuckin M, Goldman R, Bolton L, Salcido R: The clinical relevance of microbiology in acute and chronic wounds. Adv Skin Wound Care 2003;16:12–23.
29.
Bassetti C, Kallenberger A: Influence of chlorhexidine rinsing on the healing of oral mucosa and osseous lesions. J Clin Periodontol 1980;7:443–456.
30.
Nissen NN, Polverini PJ, Koch AE, Volin MV, Gamelli RL, DiPietro LA: Vascular endothelial growth factor mediates angiogenic activity during the proliferative phase of wound healing. Am J Pathol 1998;152:1445–1452.
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2012
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