We have investigated the topography of particle-laden macrophages in the pulmonary tissue of CD-I mice after intratracheal instillation of a suspension of 250 µg of calcium tungstate. The mice were sacrificed 1,3,7 and 14 days after the particle deposition. Lung fragments were studied by scanning electron microscopy (SEM) coupled with X-ray microanalysis that allowed in situ elemental identification of tungsten in the lungs. Tungsten-positive macrophages were distinctly located in the lungs of mice sacrificed at 1-3 days when compared with samples from mice killed 7-14 days after the calcium tungstate instillation. At 1-3 days, the tungsten-carrying macrophages were accumulated near the terminal bronchioles whereas they were seen predominantly in the alveolar ducts and sacs in the 7- to 14-day groups of mice. This suggests that during pulmonary inflammation there is a redistribution of the particle-containing macrophages throughout the deep lung tissue. In high-magnification SEM views, we observed that the tungsten-positive macrophages presented numerous surface microvilli. Tungsten-laden phagocytes were detected in interalveolar fenestrae, at the so-called Kohn pores. This finding documents that the Kohn pores may be used by inflammatory cells as a pathway for the migration of phagocytes in between adjacent alveolar sacs.

Keywords:
Lung, Kohn pores, Alveolar macrophages, Tungsten, Scanning electron microscopy
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© 1993 S. Karger AG, Basel
1993
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